spoIIQ [2019-07-29 11:31:39]

component of the SpoIIIAH-SpoIIQ type III secretion system residing in the forespore membrane, required for anchoring of proteins on both sides of the sporulation septum, required for localization and stability of SpoIIE

locus: BSU_36550

pI: 4.47

mw: 30.96 kDa

protein length: 283 aa

gene length: 852 bp

function: forespore encasement by the spore coat

product: part of the transmembrane channel linking the mother cell and the forespore

essential: no

synonyms: ywnI

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,759,702 3,760,553

The protein

Catalyzed reaction/ biological activity

• required for forespore encasement by the spore coat [Pubmed|22171814]
• required for the recruitment of [protein|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|SpoIIIAH] to the [SW|sporulation] septum on the mother cell side [Pubmed|23834622]
• required for the proper localization and stability of [protein|7C8DFE00A2B2B30CC8BEB35055D92CC2E4128F3A|SpoIIE] [Pubmed|26929302]

Structure

• [PDB|3UZO]; [PDB|3TUF] (the [protein|3282D2C25468776881778006F182FCF322C4821D|SpoIIQ]-[protein|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|SpoIIIAH] pore forming complex) [Pubmed|22431604,22431613]

[SW|Localization]

• membrane protein, forms a transmembrane channel linking the mother cell and the forespore (with [protein|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|SpoIIIAH]) [Pubmed|22431604,22431613,22171814]
• septal membranes on the forespore side [Pubmed|27381174]
• proper [SW|localization] requires [protein|00BDD4E3370DB3A9F4D75FE265A2A8DF05185CCC|SpoIIIAH], [protein|AA519F0D7DC94165C2CF5F7E230502D884898B71|GerM] and degradation of septal peptidoglycan by the cell wall hydrolases [protein|AAC4BF6FA80115AB90D2B161C1D5383625953616|SpoIID] and [protein|C9817A54C8E72193280E393D7BD3375BD1751738|SpoIIP] [Pubmed|23834622]

Expression and Regulation

Operons

• genes: [gene|3282D2C25468776881778006F182FCF322C4821D|spoIIQ]
  description: [Pubmed|9140963]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|16497325,9140963], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulation

  • expressed early during sporulation in the forespore ([protein|search|SigF]) [Pubmed|16497325,15699190,9140963]

Biological materials

Mutant

• BKE36550 ([gene|3282D2C25468776881778006F182FCF322C4821D|spoIIQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTTCATCACCTCAGC, downstream forward: _UP4_TAATGAAGAAAACGTCTATC
• BKK36550 ([gene|3282D2C25468776881778006F182FCF322C4821D|spoIIQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTTCATCACCTCAGC, downstream forward: _UP4_TAATGAAGAAAACGTCTATC

References

Reviews

• 23944268,25105965

Original publications

• 18812514,15752199,18077456,18485064,15574594,15044948,15882622,19609349,9140963,20444098,17121846,18160039,21097616,16497325,15699190,22431613,22171814,23834622,22431604,23859254,25356555,26929302,27381174